Miachon S, Biol M C, Cier J F, Chardonnet Y
C R Seances Soc Biol Fil. 1977;171(4):804-8.
A culture obtained from rat duodenal smooth muscle layer is described. The cells are isolated by trypsinization (0.2 %) and the medium used for culture is either MEM with glutamine and non essentiel AA, or RPMI, both containing 10 % foetal calf serum. The cell culture contains both smooth muscle cells and fibroblasts in proportions varying with the age of the culture. At day 6, cell differenciation is important. At day 12, when the cells and confluent, the majority of the cells are fibroblasts. Although it is difficult, the transfer of cells is possible at least twice.
本文描述了一种从大鼠十二指肠平滑肌层获得的培养物。通过胰蛋白酶消化（0.2%）分离细胞，用于培养的培养基为含有谷氨酰胺和非必需氨基酸的MEM培养基，或RPMI培养基，两者均含有10%的胎牛血清。细胞培养物中平滑肌细胞和成纤维细胞的比例随培养时间而变化。在第6天，细胞分化明显。在第12天，当细胞汇合时，大多数细胞是成纤维细胞。尽管有困难，但细胞至少可以传代两次。
