产生微小RNA的酶Dicer1对斑马鱼的发育至关重要。

The microRNA-producing enzyme Dicer1 is essential for zebrafish development.

作者信息

Wienholds Erno, Koudijs Marco J, van Eeden Freek J M, Cuppen Edwin, Plasterk Ronald H A

机构信息

Department of Functional Genomics, Hubrecht Laboratory, 3584 CT Utrecht, The Netherlands.

出版信息

Nat Genet. 2003 Nov;35(3):217-8. doi: 10.1038/ng1251. Epub 2003 Oct 5.

DOI:10.1038/ng1251

PMID:14528306

Abstract

MicroRNAs (miRNAs) are produced by the Dicer1 enzyme; the role of Dicer1 in vertebrate development is unknown. Here we report target-selected inactivation of the dicer1 gene in zebrafish. We observed an initial build-up of miRNA levels, produced by maternal Dicer1, in homozygous dicer1 mutants, but miRNA accumulation stopped after a few days. This resulted in developmental arrest around day 10. These results indicate that miRNA-producing Dicer1 is essential for vertebrate development.

摘要

微小RNA（miRNA）由Dicer1酶产生；Dicer1在脊椎动物发育中的作用尚不清楚。在此，我们报告了斑马鱼中dicer1基因的靶向选择性失活。我们观察到，在纯合dicer1突变体中，由母源性Dicer1产生的miRNA水平最初会积累，但几天后miRNA积累就停止了。这导致在第10天左右发育停滞。这些结果表明，产生miRNA的Dicer1对脊椎动物发育至关重要。