Dicer对小鼠发育至关重要。

Dicer is essential for mouse development.

作者信息

Bernstein Emily, Kim Sang Yong, Carmell Michelle A, Murchison Elizabeth P, Alcorn Heather, Li Mamie Z, Mills Alea A, Elledge Stephen J, Anderson Kathryn V, Hannon Gregory J

机构信息

Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA.

出版信息

Nat Genet. 2003 Nov;35(3):215-7. doi: 10.1038/ng1253. Epub 2003 Oct 5.

DOI:10.1038/ng1253

PMID:14528307

Abstract

To address the biological function of RNA interference (RNAi)-related pathways in mammals, we disrupted the gene Dicer1 in mice. Loss of Dicer1 lead to lethality early in development, with Dicer1-null embryos depleted of stem cells. Coupled with our inability to generate viable Dicer1-null embryonic stem (ES) cells, this suggests a role for Dicer, and, by implication, the RNAi machinery, in maintaining the stem cell population during early mouse development.

摘要

为了研究RNA干扰（RNAi）相关途径在哺乳动物中的生物学功能，我们在小鼠中破坏了Dicer1基因。Dicer1的缺失导致发育早期死亡，Dicer1基因敲除的胚胎中干细胞缺失。再加上我们无法产生有活力的Dicer1基因敲除胚胎干细胞（ES细胞），这表明Dicer以及与之相关的RNAi机制在小鼠早期发育过程中维持干细胞群体方面发挥了作用。