Lucke M, Schmidmaier G, Sadoni S, Wildemann B, Schiller R, Stemberger A, Haas N P, Raschke M
Department of Trauma and Reconstructive Surgery, Charité, Humboldt-University of Berlin, Germany.
J Biomed Mater Res B Appl Biomater. 2003 Oct 15;67(1):593-602. doi: 10.1002/jbm.b.10051.
Infection related to osteosynthesis often has dramatic consequences for the patient. Prolonged hospitalization with systemic antibiotic therapy, several revision procedures, possible amputation, and even death may occur. To investigate the pathology of infection in orthopedic surgery, a new rat model of implant related osteomyelitis was developed. Three different concentrations (10(6), 10(3), and 10(2) colony-forming units (CFU)/10 microl) of Staphylococcus aureus were inoculated into the tibial medullary cavity with simultaneous insertion of a titanium Kirschner wire. Controls received phosphate-buffered saline (PBS). Each group consisted of 10 animals. Animals were followed for 4 weeks until sacrifice. X-rays of the tibiae were taken weekly, blood counts were analyzed, and body temperature and weight were determined. After sacrifice, infection was evaluated by histological and microbiological investigations. All animals inoculated with Staph. aureus in either concentration developed microbiological, histological, and radiological signs of osteomyelitis in correlation to the amount of inoculated bacteria. X-rays clearly revealed osseous destruction after 14 days with progression of osteomyelitis during the following weeks. CFU/g bone and bone weight after sacrifice showed dependence on the amount of inoculated CFU. The histological results confirmed the radiological findings. No significant changes in blood counts, body weight, and body temperature between the groups could be observed. The results demonstrate that it is possible to develop a model of implant-related osteomyelitis in rats with dependence on the amount of inoculated bacteria. No other promoters of infection besides intramedullary insertion of titanium Kirschner wires were used in this model.
与骨固定相关的感染常常给患者带来严重后果。可能会出现因全身使用抗生素治疗而导致的长期住院、多次翻修手术、截肢甚至死亡。为了研究骨科手术中感染的病理情况，建立了一种新的与植入物相关的骨髓炎大鼠模型。将三种不同浓度（10⁶、10³和10²菌落形成单位（CFU）/10微升）的金黄色葡萄球菌接种到胫骨骨髓腔中，同时插入一根钛克氏针。对照组接受磷酸盐缓冲盐水（PBS）。每组有10只动物。对动物进行4周的跟踪观察直至处死。每周对胫骨进行X线检查，分析血常规，并测定体温和体重。处死动物后，通过组织学和微生物学检查评估感染情况。所有接种了任何一种浓度金黄色葡萄球菌的动物均出现了与接种细菌数量相关的骨髓炎微生物学、组织学和放射学表现。X线在14天后清晰显示骨质破坏，在接下来的几周内骨髓炎不断进展。处死动物后每克骨的CFU和骨重量显示出对接种CFU数量的依赖性。组织学结果证实了放射学检查结果。各组之间在血常规、体重和体温方面未观察到显著变化。结果表明，有可能建立一种依赖于接种细菌数量的大鼠植入物相关骨髓炎模型。该模型除了在骨髓腔内插入钛克氏针外，未使用其他感染促进因素。
