Ebsworth P, Bryan J H, Foley D H
International SOS, Ji Puri Sakti No.10, Cipete, Jakarta Selatan, Indonesia.
J Am Mosq Control Assoc. 2001 Sep;17(3):181-5.
We surveyed the larval habitats of members of the Anopheles punctulatus group of mosquitoes on Niolam (Lihir) Island, Papua New Guinea. Identification of this group was undertaken by polymerase chain reaction-restriction fragment length polymorphism analysis of the amplified internal transcribed spacer unit 2 of rDNA, because morphologic separation of member species is unreliable. The most widespread malaria vector species and their most common larval habitats were identified to aid source-reduction programs for malaria control. The most ubiquitous species was An. punctulatus, followed by An. farauti no. 2. then An. farauti s.s. Anopheles punctulatus has increased relative to An. farauti s.l. since the start of development projects on Lihir Island. The most common larval habitats were shallow temporary pools with clay substrate and with plants or floatage. These habitats, mostly encountered alongside poorly drained roads, may be increased by development projects.
我们对巴布亚新几内亚尼奥拉姆（利希尔）岛上致倦库蚊组蚊虫的幼虫栖息地进行了调查。由于该组成员物种的形态分离不可靠，因此通过对核糖体DNA扩增的内部转录间隔区2进行聚合酶链反应-限制性片段长度多态性分析来进行该组的鉴定。确定了最广泛分布的疟疾传播媒介物种及其最常见的幼虫栖息地，以协助开展减少疟疾传染源的控制项目。分布最广的物种是致倦库蚊，其次是二号费氏按蚊，然后是指名费氏按蚊。自利希尔岛开发项目启动以来，致倦库蚊相对于费氏按蚊复合组有所增加。最常见的幼虫栖息地是带有粘土基质以及植物或漂浮物的浅临时水池。这些栖息地大多出现在排水不良的道路旁，开发项目可能会增加此类栖息地。
