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同时降解氯代芳烃和甲基芳烃的施氏假单胞菌CLN100的多相特征:该物种内的一个新基因组变种

Polyphasic characterization of Pseudomonas stutzeri CLN100 which simultaneously degrades chloro- and methylaromatics: a new genomovar within the species.

作者信息

García-Valdés Elena, Castillo María M, Bennasar Antonio, Guasp Caterina, Cladera Aina M, Bosch Rafael, Engesser Karl H, Lalucat Jorge

机构信息

Department de Biologia, Microbiologia, Universitat de les Illes Balears, and Institut Mediterrani d'Estudis Avanqats (CSIC-UIB), Palma de Mallorca, Spain.

出版信息

Syst Appl Microbiol. 2003 Sep;26(3):390-403. doi: 10.1078/072320203322497428.

Abstract

Strain CLN100 was isolated after enrichment on mineral medium with chloronaphthalene as the only carbon and energy source. It was able to use simultaneously and productively chloro- and methyl-derivatives of naphthalene and salicylate through a chromosomally encoded meta pathway. Phenotypic, chemotaxonomic and genotypic characterization classified strain CLN100 as a member of the species Pseudomonas stutzeri. DNA-DNA hybridizations, 16S rDNA, gyrB, rpoD sequences, and molecular fingerprinting indicate that strain CLN100 is a representative of a new genomovar (genomovar 10) within the species.

摘要

菌株CLN100是在以氯萘作为唯一碳源和能源的矿物培养基上富集培养后分离得到的。它能够通过染色体编码的间位途径同时高效利用萘和水杨酸盐的氯代及甲基衍生物。通过表型、化学分类学和基因型特征鉴定,菌株CLN100被归类为斯氏假单胞菌物种的一员。DNA-DNA杂交、16S rDNA、gyrB、rpoD序列以及分子指纹图谱表明,菌株CLN100是该物种内一个新基因组变种(基因组变种10)的代表。

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