Zhang Fan, She Yue-Hui, Banat Ibrahim M, Chai Lu-Jun, Huang Liu-Qin, Yi Shao-Jin, Wang Zheng-Liang, Dong Hai-Liang, Hou Du-Jie
The Key Laboratory of Marine Reservoir Evolution and Hydrocarbon Accumulation Mechanism, Ministry of Education, China; School of Energy Resources, China University of Geosciences (Beijing), Beijing, 100083, China.
Microbiologyopen. 2014 Aug;3(4):446-56. doi: 10.1002/mbo3.179. Epub 2014 Jun 2.
Oil reservoirs are specific habitats for the survival and growth of microorganisms in general. Pseudomonas stutzeri which is believed to be an exogenous organism inoculated into oil reservoirs during the process of oil production was detected frequently in samples from oil reservoirs. Very little is known, however, about the distribution and genetic structure of P. stutzeri in the special environment of oil reservoirs. In this study, we collected 59 P. stutzeri 16S rRNA gene sequences that were identified in 42 samples from 25 different oil reservoirs and we isolated 11 cultured strains from two representative oil reservoirs aiming to analyze the diversity and genomovar assignment of the species in oil reservoirs. High diversity of P. stutzeri was observed, which was exemplified in the detection of sequences assigned to four known genomovars 1, 2, 3, 20 and eight unknown genomic groups of P. stutzeri. The frequent detection and predominance of strains belonging to genomovar 1 in most of the oil reservoirs under study indicated an association of genomovars of P. stutzeri with the oil field environments.
油藏总体上是微生物生存和生长的特定栖息地。斯氏假单胞菌被认为是在石油生产过程中接种到油藏中的一种外源生物，在油藏样品中经常被检测到。然而，对于斯氏假单胞菌在油藏特殊环境中的分布和遗传结构知之甚少。在本研究中，我们收集了在来自25个不同油藏的42个样品中鉴定出的59个斯氏假单胞菌16S rRNA基因序列，并从两个代表性油藏中分离出11株培养菌株，旨在分析该物种在油藏中的多样性和基因组分类。观察到斯氏假单胞菌具有高度多样性，这体现在检测到属于四个已知基因组分类1、2、3、20以及八个未知斯氏假单胞菌基因组群的序列。在所研究的大多数油藏中，属于基因组分类1的菌株频繁被检测到且占主导地位，这表明斯氏假单胞菌的基因组分类与油田环境有关。
