Esipov Roman S, Chupova Larisa A, Shvets Sergey V, Chuvikovsky Dmitry V, Gurevich Alexandr I, Muravyova Tatyana I, Miroshnikov Anatoly I
Shemyakin Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997 Russia.
Protein Pept Lett. 2003 Aug;10(4):404-11. doi: 10.2174/0929866033478807.
The plasmid DNA pERilox4 containing the gene of the recombinant protein, which included the leader sequence and the oxytocinoyl lysine tetramer, was constructed. The high level of gene expression in E. coli was achieved. The method for purification of the recombinant protein and its isolation in the soluble form was developed. The conditions for digestion of the hybrid protein by trypsin and carboxypeptidase B were matched. The effective method for transformation of oxytocinic acid to oxytocin was worked out. The scheme suggested allowed obtaining oxytocin in high yield.
构建了含有重组蛋白基因（包括前导序列和催产素赖氨酸四聚体）的质粒DNA pERilox4。实现了该基因在大肠杆菌中的高水平表达。开发了重组蛋白的纯化方法及其以可溶形式的分离方法。匹配了用胰蛋白酶和羧肽酶B消化杂合蛋白的条件。制定了将催产酸转化为催产素的有效方法。所建议的方案能够高产获得催产素。
