Mal'tsev K V, Shvets S V, Luk'ianova N A, Grigor'ev A V, Miroshnikov A I
Bioorg Khim. 1996 Apr;22(4):252-5.
Various methods for obtaining oxytocin from its recombinant precursor oxytocinoyllysine were studied. For splitting off the C-terminal lysine residue in oxytocinoyllysine, the carboxypeptidase B and an analogous carboxypeptidase from king crab hepatopancreas were used. Ammonolysis of oxytocinic acid methyl ester proved to be the most efficient method for the last stage of the oxytocin preparation. Reversed-phase HPLC was used for the product analysis at each stage of the recombinant oxytocinoyllysine conversion into oxytocin.
研究了从重组前体催产素赖氨酸获得催产素的各种方法。为了去除催产素赖氨酸中的C末端赖氨酸残基，使用了羧肽酶B和来自帝王蟹肝胰腺的类似羧肽酶。催产素酸甲酯的氨解被证明是催产素制备最后阶段最有效的方法。在重组催产素赖氨酸转化为催产素的每个阶段，都使用反相高效液相色谱法进行产物分析。
