一种用于空肠弯曲菌、单核细胞增生李斯特菌和大肠杆菌同时进行菌落计数和最大可能数（MPN）计数的6×6滴板法。
A 6 x 6 drop plate method for simultaneous colony counting and MPN enumeration of Campylobacter jejuni, Listeria monocytogenes, and Escherichia coli.
作者信息
Chen Chin Yi, Nace Gary W, Irwin Peter L
机构信息
Eastern Regional Research Center, Agricultural Research Service, United States Department of Agriculture, 600 E. Mermaid Lane, Wyndmoor, PA 19038, USA.
出版信息
J Microbiol Methods. 2003 Nov;55(2):475-9. doi: 10.1016/s0167-7012(03)00194-5.
Abstract
A protocol was developed using 96-well plates and multichannel pipettes for serial dilutions, followed by drop plating on agar in a 6 x 6 format. This protocol permits simultaneous plating of six dilutions which greatly decreases the number of plates utilized thereby saving incubator space for organisms such as Campylobacter which require unique environmental conditions.
摘要
开发了一种使用96孔板和多通道移液器进行系列稀释的方案，然后以6×6的形式在琼脂上滴涂。该方案允许同时接种六种稀释液，从而大大减少了所用平板的数量，从而为需要独特环境条件的弯曲杆菌等微生物节省了培养箱空间。
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