Spangrude G J
Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana.
Int J Cell Cloning. 1992 Sep;10(5):277-85. doi: 10.1002/stem.5530100505.
Mouse hematopoietic stem cells can be enriched from adult bone marrow by a number of methods. The resulting cell populations are heterogeneous in function, suggesting a complex organizational structure within the stem cell compartment. Several assays can be applied to the study of early stages of hematopoiesis; however clonal assays for long-term repopulation, the most critical operational definition of hematopoietic stem cells, are lacking. Further complicating the prospect of understanding early hematopoiesis is the finding that genetic variations among laboratory strains of mice lead to major differences in phenotypic and functional characteristics of hematopoietic stem cells. Application to the human situation of the methodology developed for stem cell isolation and characterization in the mouse will be hampered by the possibility of genetic variations among human subjects and the lack of a well-characterized assay system to detect and quantify cells capable of long-term repopulation of irradiated recipients.
小鼠造血干细胞可通过多种方法从成年骨髓中富集。所得到的细胞群体在功能上具有异质性，这表明干细胞区室内存在复杂的组织结构。有几种检测方法可用于造血早期阶段的研究；然而，缺乏用于长期重建的克隆检测方法，而长期重建是造血干细胞最关键的操作定义。进一步使理解早期造血过程变得复杂的是，研究发现小鼠实验室品系之间的基因变异会导致造血干细胞的表型和功能特征出现重大差异。将从小鼠干细胞分离和表征中开发的方法应用于人类情况时，会受到人类个体之间基因变异的可能性以及缺乏用于检测和量化能够长期重建受辐照受体的细胞的特征明确的检测系统的阻碍。
