Correa P N, Axelrad A A
Department of Anatomy and Cell Biology, Faculty of Medicine, University of Toronto, Ontario, Canada.
Int J Cell Cloning. 1992 Sep;10(5):286-91. doi: 10.1002/stem.5530100506.
Retinyl acetate (RA) dramatically increased the production of early (d16) erythroid colonies in vitro by circulating human progenitor cells growing in an improved serum-free (SF) medium. In the absence of either erythropoietin (Epo) or insulin-like growth factor I (IGF-I), RA alone was able to induce the hemoglobinization of cells in these erythroid colonies. RA synergized with Epo or with IGF-I to yield increased numbers of well-hemoglobinized early colonies. In the presence of defined burst promoting activity (BPA) provided by recombinant human interleukin 3 (rHuIL-3) and hemin, RA and all-trans-retinoic acid (ATRA) were identical with respect to their differentiation-inducing function for early erythroid colonies. ATRA increased the number of these colonies in a concentration-dependent manner, with maximal stimulation (3.5-fold) occurring at 30 nM in the presence of 5.5 ng/ml IL-3, 0.1 mM hemin, 3.0 U/ml Epo and 30 nM IGF-I. This appears to be the first demonstration of erythropoietic activity of two metabolic derivatives of vitamin A in SF medium.
醋酸视黄酯（RA）显著增加了体外循环人祖细胞在改良无血清（SF）培养基中生长时早期（第16天）红系集落的生成。在缺乏促红细胞生成素（Epo）或胰岛素样生长因子I（IGF-I）的情况下，单独的RA就能诱导这些红系集落中的细胞血红蛋白化。RA与Epo或IGF-I协同作用，产生数量增加且血红蛋白化良好的早期集落。在重组人白细胞介素3（rHuIL-3）和血红素提供的特定爆式促进活性（BPA）存在的情况下，RA和全反式维甲酸（ATRA）对早期红系集落的诱导分化功能相同。ATRA以浓度依赖的方式增加这些集落的数量，在存在5.5 ng/ml IL-3、0.1 mM血红素、3.0 U/ml Epo和30 nM IGF-I的情况下，30 nM时出现最大刺激（3.5倍）。这似乎是首次证明维生素A的两种代谢衍生物在SF培养基中的促红细胞生成活性。
