Dupree W G, Bradford H B
Division of Laboratories, Louisiana Dept of Health and Hospitals, New Orleans.
J La State Med Soc. 1992 Aug;144(8):379-82.
Clinical laboratory mycobacteriology has traditionally involved long delays for results, primarily due to the slow growth rate of most members of the genus Mycobacterium. Several new methodologies are now available that enable dramatic reductions in turn-around times. These methodologies include the Bactec TB System for isolation and drug susceptibility testing of Mycobacterium tuberculosis, the use of DNA probes, and high performance liquid chromatography (HPLC) for the identification of mycobacterial isolates. The spread of multidrug-resistant tuberculosis has made it imperative that laboratories take advantage of these new methodologies whenever possible. The Mycobacteriology Unit of the Louisiana Office of Public Health's Division of Laboratories has pioneered the use of these methodologies in a public health laboratory setting. Great reductions in turn-around times have been achieved and further reductions are expected as the methods are refined and adapted to the needs of a high-volume public health laboratory.
传统上，临床实验室分枝杆菌学的检测结果等待时间很长，主要是因为分枝杆菌属的大多数成员生长速度缓慢。现在有几种新方法可以大幅缩短周转时间。这些方法包括用于结核分枝杆菌分离和药敏试验的Bactec TB系统、DNA探针的使用以及用于鉴定分枝杆菌分离株的高效液相色谱法（HPLC）。耐多药结核病的传播使得实验室必须尽可能利用这些新方法。路易斯安那州公共卫生部实验室司的分枝杆菌学部门率先在公共卫生实验室环境中使用这些方法。周转时间已大幅缩短，随着这些方法的完善并适应大容量公共卫生实验室的需求，预计还会进一步缩短。
