Koulman Albert, Kubbinga Marlies E, Batterman Sieb, Woerdenbag Herman J, Pras Niesko, Woolley Jack G, Quax Wim J
Department of Pharmaceutical Biology, GUIDE (Groningen University Institute for Drug Exploration), Groningen, The Netherlands.
Planta Med. 2003 Aug;69(8):733-8. doi: 10.1055/s-2003-42776.
In the roots of Anthriscus sylvestris 12 different lignans were detected. Arctigenin, dimethylmatairesinol, dimethylthujaplicatin, podophyllotoxin, 7-hydroxyyatein and 7-hydroxyanhydropodorhizol have not been previously reported to be present in A. sylvestris. In the cell suspension cultures, which were initiated for this study, trace amounts of deoxypodophyllotoxin could be detected. With these cell suspension cultures we carried out feeding experiments using deoxypodophyllotoxin, yatein and, anhydropodorhizol. Yatein had a toxic effect on the cell cultures and was, like anhydropodorhizol, not converted into any detectable product. Deoxypodophyllotoxin, in contrast, was converted into podophyllotoxin, yielding significantly higher concentration than measured in whole plants.
在峨参的根部检测到了12种不同的木脂素。牛蒡子苷元、二甲基芝麻素、二甲基苏合香素、鬼臼毒素、7-羟基叶下珠素和7-羟基脱水山荷叶素此前尚未报道存在于峨参中。在为本研究启动的细胞悬浮培养物中，可检测到痕量的脱氧鬼臼毒素。利用这些细胞悬浮培养物，我们使用脱氧鬼臼毒素、叶下珠素和脱水山荷叶素进行了饲喂实验。叶下珠素对细胞培养物具有毒性作用，并且与脱水山荷叶素一样，未转化为任何可检测到的产物。相比之下，脱氧鬼臼毒素转化为了鬼臼毒素，其产生的浓度明显高于在整株植物中测得的浓度。
