Koulman Albert, Batterman Sieb, van Putten Freeke M, Bos Rein, Quax Wim J
Department of Pharmaceutical Biology, GUIDE (Groningen University Institute for Drug Exploration), University of Groningen, Groningen, The Netherlands.
Planta Med. 2003 Oct;69(10):959-61. doi: 10.1055/s-2003-45110.
In a previous study we have shown that different populations of Anthriscus sylvestris (L.) Hoffm. (Apiaceae) yield significantly different lignan profiles. In this study we collected the seeds of A. sylvestris from 4 locations, one in England and three in The Netherlands. The seeds germinated and were grown under identical laboratory conditions. After 5 months the plants were harvested and the lignan profile in the roots and aerial parts was analysed using GC-MS. The plants from the seeds of the 4 locations showed several significant phenotypic differences in, for instance, dry-weight. However the 4 groups did not differ significantly in their lignan profile in their roots. The lignans in the roots of A. sylvestris, deoxypodophyllotoxin, yatein and anhydropodorhizol reached, on average, equal amounts in all 4 groups. Within the 4 groups, however, a large quantitative variation in lignan profile between the individual plants was observed, pointing to within-population genetic differences between individual plants.
在之前的一项研究中，我们已经表明，不同种群的峨参（Anthriscus sylvestris (L.) Hoffm.，伞形科）产生的木脂素谱有显著差异。在本研究中，我们从4个地点收集了峨参种子，1个在英国，3个在荷兰。种子发芽后在相同的实验室条件下生长。5个月后收获植株，使用气相色谱 - 质谱联用仪（GC-MS）分析根和地上部分的木脂素谱。来自4个地点种子的植株在例如干重等方面表现出几个显著的表型差异。然而，这4组植株根中的木脂素谱没有显著差异。峨参根中的木脂素，脱氧鬼臼毒素、叶因和脱水足叶草醇，在所有4组中平均含量相等。然而，在这4组中，观察到个体植株之间木脂素谱存在很大的数量差异，这表明个体植株之间存在种群内遗传差异。
