从活性污泥中分离出的非絮凝细菌间的共凝聚作用。
Coaggregation among nonflocculating bacteria isolated from activated sludge.
作者信息
Malik Anushree, Sakamoto Masashi, Hanazaki Shohei, Osawa Masamitsu, Suzuki Takanori, Tochigi Masaki, Kakii Kazuo
机构信息
Department of Applied Chemistry, Faculty of Engineering, Utsunomiya University, Utsunomiya 321-8585, Japan.
出版信息
Appl Environ Microbiol. 2003 Oct;69(10):6056-63. doi: 10.1128/AEM.69.10.6056-6063.2003.
Thirty-two strains of nonflocculating bacteria isolated from sewage-activated sludge were tested by a spectrophotometric assay for their ability to coaggregate with one other in two-membered systems. Among these strains, eight showed significant (74 to 99%) coaggregation with Acinetobacter johnsonii S35 while only four strains coaggregated, to a lesser extent (43 to 65%), with Acinetobacter junii S33. The extent and pattern of coaggregation as well as the aggregate size showed good correlation with cellular characteristics of the coaggregating partners. These strains were identified by sequencing of full-length 16S rRNA genes. A. johnsonii S35 could coaggregate with strains of several genera, such as Oligotropha carboxidovorans, Microbacterium esteraromaticum, and Xanthomonas spp. The role of Acinetobacter isolates as bridging organisms in multigeneric coaggregates is indicated. This investigation revealed the role of much-neglected nonflocculating bacteria in floc formation in activated sludge.
通过分光光度法检测了从污水活性污泥中分离出的32株非絮凝细菌在二元体系中彼此共聚集的能力。在这些菌株中，8株与约翰逊不动杆菌S35表现出显著的（74%至99%）共聚集，而只有4株与琼氏不动杆菌S33的共聚集程度较低（43%至65%）。共聚集的程度和模式以及聚集体大小与共聚集伙伴的细胞特征显示出良好的相关性。通过对全长16S rRNA基因进行测序对这些菌株进行了鉴定。约翰逊不动杆菌S35可与多个属的菌株共聚集，如食羧寡养菌、酯芳香微杆菌和黄单胞菌属。表明不动杆菌分离株在多属共聚集体中作为桥梁生物的作用。这项研究揭示了被忽视的非絮凝细菌在活性污泥絮凝形成中的作用。
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