Escobar-Sánchez Monserrat, Carrasco-Navarro Ulises, Juárez-Castelán Carmen, Lozano-Aguirre Beltrán Luis, Pérez-Chabela M Lourdes, Ponce-Alquicira Edith
Departamento de Biotecnología, Universidad Autónoma Metropolitana Unidad Iztapalapa, Ciudad de México 09340, Mexico.
Cinvestav, Departamento de Genética y Biología Molecular, Ciudad de México 07360, Mexico.
Foods. 2022 Dec 22;12(1):46. doi: 10.3390/foods12010046.
1101 was identified by using 16S rRNA and MALDI-Biotyper. The strain was exposed to conditions that resemble the gastrointestinal tract (GT) to evaluate its probiotic properties. That included the growth kinetics, proteolytic and inhibitory activities within a pH range, survival at low pH and in the presence of bile salts, antagonistic activity, cell-adhesion properties, and antibiotic resistance. The evaluation was followed by a genomic and proteomic analysis that involved the identification of proteins obtained under control and gastrointestinal conditions. The strain showed antagonistic activity against Gram-negative and Gram-positive bacteria, high resistance to acidity (87% logarithmic survival rate, pH 2) and bile salts (99% logarithmic survival rate, 0.5% /), and hydrophobic binding, as well as sensitivity to penicillin, amoxicillin, and chloramphenicol. On the other hand, 1101 has a genome size of 1.76 Mbp, with 1754 coding sequences, 55 rRNAs, and 33 tRNAs. The proteomic analysis showed that 120 proteins were involved in mechanisms in which the strain senses the effects of acid and bile salts. Moreover, the strain produces at least one lytic enzyme (-acetylmuramoyl-L-alanine amidase; 32 kDa) that may be related to the antimicrobial activity. Therefore, proteins identified might be a key factor when it comes to the adaptation of 1101 into the GT and associated with its technological and probiotic properties.
通过16S rRNA和基质辅助激光解吸电离生物分型法鉴定出1101菌株。将该菌株置于模拟胃肠道（GT）的条件下，以评估其益生菌特性。这包括生长动力学、在一定pH范围内的蛋白水解和抑制活性、在低pH值和胆盐存在下的存活率、拮抗活性、细胞黏附特性以及抗生素抗性。评估之后进行了基因组和蛋白质组分析，其中涉及鉴定在对照条件和胃肠道条件下获得的蛋白质。该菌株对革兰氏阴性菌和革兰氏阳性菌表现出拮抗活性，对酸性环境（pH 2时对数存活率为87%）和胆盐（0.5%时对数存活率为99%）具有高抗性，具有疏水结合能力，并且对青霉素、阿莫西林和氯霉素敏感。另一方面，1101菌株的基因组大小为1.76 Mbp，有1754个编码序列、55个rRNA和33个tRNA。蛋白质组分析表明，有120种蛋白质参与该菌株感知酸和胆盐影响的机制。此外，该菌株产生至少一种可能与抗菌活性相关的裂解酶（N-乙酰胞壁酰-L-丙氨酸酰胺酶；32 kDa）。因此，鉴定出的蛋白质可能是1101菌株适应胃肠道并与其技术和益生菌特性相关的关键因素。
