Vliegenthart J F
Bijvoet Center, Department Bio-Organic Chemistry, Utrecht University, Utrecht, The Netherlands.
Adv Exp Med Biol. 2001;491:133-40.
In this report, an overview is presented of studies on the three-dimensional structure in solution of glycoproteins. The investigations were carried out by using high resolution NMR spectroscopy in conjunction with molecular dynamics calculations and molecular modelling. For pine apple stem bromelain, ribonuclease 2 and the free alpha-subunit of human chorionic gonadotropin it could be shown that intramolecular interactions between carbohydrate and protein exist. Glycosylation sites are rather unique structural entities in glycoproteins, because of the local spatial arrangements. This is reflected in different intramolecular interactions between carbohydrate and protein.
在本报告中，对糖蛋白溶液三维结构的研究进行了概述。研究采用高分辨率核磁共振光谱结合分子动力学计算和分子建模来开展。对于菠萝茎菠萝蛋白酶、核糖核酸酶2和人绒毛膜促性腺激素游离α亚基，已证实碳水化合物与蛋白质之间存在分子内相互作用。由于局部空间排列，糖基化位点在糖蛋白中是相当独特的结构实体。这反映在碳水化合物与蛋白质之间不同的分子内相互作用上。
