Barb Adam W, Borgert Andrew J, Liu Mian, Barany George, Live David
Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia, USA.
Methods Enzymol. 2010;478:365-88. doi: 10.1016/S0076-6879(10)78018-6.
Glycoproteins are a major class of glycoconjugates displaying a variety of mutual interactions between glycan and protein moieties that ultimately affect molecular organization. Modulation of the pendant glycan structures is important in tuning the functions of glycoproteins. Here we discuss structural aspects and some of the challenges to studying intramolecular interactions between carbohydrate and protein elements in several forms of O-linked as well as N-linked glycoproteins. These illustrate the importance of the relationship of context to function in protein glycosylation.
糖蛋白是一类主要的糖缀合物，其聚糖和蛋白质部分之间存在多种相互作用，最终影响分子结构。侧链聚糖结构的调节对于调节糖蛋白的功能很重要。在这里，我们讨论了几种O-连接和N-连接糖蛋白中碳水化合物和蛋白质元素之间分子内相互作用的结构方面以及一些研究挑战。这些说明了蛋白质糖基化中背景与功能关系的重要性。
