Mitra Aparna, Chakrabarti Jayati, Chattopadhyay Nibedita, Chatterjee Amitava
Department of Receptor Biology and Tumor Metastasis, Chittaranjan National Cancer Institute, Kolkata, India.
J Environ Pathol Toxicol Oncol. 2003;22(2):93-100. doi: 10.1615/jenvpathtoxoncol.v22.i2.20.
Matrixmetalloproteinases (MMPs) are a family of secreted or membrane-associated proteins capable of digesting extracellular matrix components. The importance of MMPs in tumor development and invasion is well known. Recent studies have strongly indicated the presence of a functional complex consisting of alpha(v)beta3 integrin, membrane type-1 metalloproteinase-2 (MT1-MMP), and tissue inhibitor of metalloproteinase-2 (TIMP-2) on tumor cell surface, which helps the activation of MMP-2. In this article, we report on the association of active MMP-2 with the membrane fraction of human cervical cancer cells. The association of MMP-2 with alpha(v)beta3 integrin and the expression of membrane type MT1-MMP strongly indicate the role of alpha(v)beta3/MT1-MMP/TIMP-2 complex in the activation of MMP-2 in cervical cancer tissue membrane fraction. The membrane-associated activated MMP-2 may have a role in the migration of tumor cells during invasion.
基质金属蛋白酶（MMPs）是一类能够消化细胞外基质成分的分泌型或膜相关蛋白。MMPs在肿瘤发生和侵袭中的重要性已广为人知。最近的研究有力地表明，肿瘤细胞表面存在一种由α（v）β3整合素、膜型-1金属蛋白酶-2（MT1-MMP）和金属蛋白酶组织抑制剂-2（TIMP-2）组成的功能复合物，该复合物有助于MMP-2的激活。在本文中，我们报道了活性MMP-2与人宫颈癌细胞膜部分的关联。MMP-2与α（v）β3整合素的关联以及膜型MT1-MMP的表达强烈表明α（v）β3/MT1-MMP/TIMP-2复合物在宫颈癌组织膜部分MMP-2激活中的作用。膜相关的活化MMP-2可能在肿瘤细胞侵袭过程中的迁移中发挥作用。
