Mohanty Aparajita, Martín Juan Pedro, González Luis Miguel, Aguinagalde Itziar
Departamento de Biología Vegetal, Escuela Técnica Superior de Ingenieros Agrónomos, Universidad Politécnica de Madrid, Ciudad Universitaria, 28040 Madrid, Spain.
Ann Bot. 2003 Dec;92(6):749-55. doi: 10.1093/aob/mcg198. Epub 2003 Oct 8.
Chloroplast DNA (cpDNA) and mitochondrial DNA (mtDNA) were studied in 24 populations of Prunus spinosa sampled across Europe. The cpDNA and mtDNA fragments were amplified using universal primers and subsequently digested with restriction enzymes to obtain the polymorphisms. Combinations of all the polymorphisms resulted in 33 cpDNA haplotypes and two mtDNA haplotypes. Strict association between the cpDNA haplotypes and the mtDNA haplotypes was detected in most cases, indicating conjoint inheritance of the two genomes. The most frequent and abundant cpDNA haplotype (C20; frequency, 51 %) is always associated with the more frequent and abundant mtDNA haplotype (M1; frequency, 84 %). All but two of the cpDNA haplotypes associated with the less frequent mtDNA haplotype (M2) are private haplotypes. These private haplotypes are phylogenetically related but geographically unrelated. They form a separate cluster on the minimum-length spanning tree.
对欧洲各地采集的24个黑刺李种群的叶绿体DNA（cpDNA）和线粒体DNA（mtDNA）进行了研究。使用通用引物扩增cpDNA和mtDNA片段，随后用限制性内切酶消化以获得多态性。所有多态性的组合产生了33种cpDNA单倍型和两种mtDNA单倍型。在大多数情况下，检测到cpDNA单倍型和mtDNA单倍型之间存在严格关联，表明这两个基因组的联合遗传。最常见且丰富的cpDNA单倍型（C20；频率为51%）总是与更常见且丰富的mtDNA单倍型（M1；频率为84%）相关联。除了两种与频率较低的mtDNA单倍型（M2）相关的cpDNA单倍型外，其余均为私有单倍型。这些私有单倍型在系统发育上相关，但在地理上不相关。它们在最小长度生成树上形成一个单独的簇。
