Departamento de Biología Vegetal, Escuela Técnica Superior de Ingenieros Agrónomos, Universidad Politécnica de Madrid, Ciudad Universitaria, 28040 Madrid, Spain.
Am J Bot. 2002 Aug;89(8):1223-8. doi: 10.3732/ajb.89.8.1223.
Chloroplast DNA diversity in Prunus spinosa, a common shrub of European deciduous forests, was assessed using the polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) technique. Thirty-two haplotypes were detected in 25 populations spread across the European continent. Ten haplotypes were shared by two or more populations, and 22 were private. The major proportion of the total cpDNA diversity (H(T) = 0.73) was located within the populations (H(S) = 0.49), and differentiation between populations was low (G(ST) = 0.33) compared with other forest species. Haplotype diversity was higher in southern Europe than in northern Europe, indicating probable localization of glacial refugia in southern Europe. The minimum-length spanning tree of haplotypes showed incongruency between the phylogeny of haplotypes and their geographic locations. This might be the result of intensive seed movements following recolonization, which thereby erased the phylogeographic structure in P. spinosa.
利用聚合酶链反应限制片段长度多态性（PCR-RFLP）技术评估了欧洲落叶林中常见灌木欧洲李的叶绿体 DNA 多样性。在分布于整个欧洲大陆的 25 个种群中检测到 32 种单倍型。有 10 种单倍型为两个或更多种群共有，22 种为特有。总 cpDNA 多样性的主要部分（H(T) = 0.73）位于种群内（H(S) = 0.49），与其他森林物种相比，种群间的分化程度较低（G(ST) = 0.33）。与北欧相比，南欧的单倍型多样性更高，表明可能在南欧存在冰川避难所。单倍型最短跨度树表明单倍型的系统发育与其地理位置之间存在不一致性。这可能是由于重新殖民化后种子的大量移动，从而消除了欧洲李中的系统地理结构。
