Iida Koji, Nobori Tsutomu, Matsumine Akihiko, Isaka Akihiko, Seto Masashi, Shiraishi Taizou, Uchida Atsumasa
Department of Orthopedic Surgery, Mie University School of Medicine, Edobashi 2-174, Tsu City, Mie Prefecture 514-8507, Japan.
Oncol Rep. 2003 Nov-Dec;10(6):1961-5.
We examined the effects of inactivation of the RB gene on chemosensitivity of human osteosarcoma cell lines, using the MTT assay and calculating the inhibition index. Although the human osteosarcoma cell lines HOS and MG63 have a wild-type RB gene, SaOS-2 and OSrb (established from retinoblastoma patient) have no active RB gene. We used these 4 cell lines in growth inhibition assays for doxorubicin, cisplatin and methotrexate, and assessed the chemosensitivity. In the growth inhibition assay for methotrexate, cell lines lacking an active RB gene were more resistant than cell lines with an active RB gene.
我们使用MTT法并计算抑制指数，研究了RB基因失活对人骨肉瘤细胞系化学敏感性的影响。人骨肉瘤细胞系HOS和MG63具有野生型RB基因，而SaOS-2和OSrb（源自视网膜母细胞瘤患者）没有活性RB基因。我们将这4种细胞系用于阿霉素、顺铂和甲氨蝶呤的生长抑制试验，并评估化学敏感性。在甲氨蝶呤的生长抑制试验中，缺乏活性RB基因的细胞系比具有活性RB基因的细胞系更具抗性。
