Jiang Wen G, Ablin Richard, Douglas-Jones Anthony, Mansel Robert E
University Department of Surgery, University of Wales College of Medicine, Cardiff, CF14 4XN, UK.
Oncol Rep. 2003 Nov-Dec;10(6):2039-44.
Implicated in several physiologic processes, including tumour-host, some transglutaminases (TGase) have been noted to play a regulatory role in the extracellular matrix in cell adhesion and migration of cancer cells. This study sought to determine the level of expression of TGases and their possible clinical significance in a cohort of human breast cancer patients using RT-PCR and quantitative RT-PCR. Normal breast tissues generally expressed low levels of TGases-1, 2, 3 and 7, and higher levels of TGases-4, 5 and plasma TGase (FXIII). Significantly increased levels of transcripts of TGases-4 and 7, and significantly lower levels of FXIII were seen in tumour tissues (n=110) compared with normal mammary tissues (n=27), p=0.05, 0.04 and 0.05, respectively. Node positive tumours exhibited significantly higher levels of TGase-2 and lower levels of TGase-3 (p=0.05 and 0.046, respectively). The lowest levels of TGases-3 and 7 were seen in patients with metastatic disease, and TGase-3 in patients who died of breast cancer, compared with those who remained disease-free (median follow-up 72 months). Higher levels of TGases-4 and 5 were noted in patients with local recurrence. Breast cancer displays an aberrant expression of TGases, wherein the levels of TGases-2, 3 and 7 have a relationship with node involvement and patient outcome.
一些转谷氨酰胺酶（TGase）参与了包括肿瘤与宿主关系在内的多种生理过程，已被发现它们在细胞外基质中对癌细胞的黏附和迁移起着调节作用。本研究旨在通过逆转录聚合酶链反应（RT-PCR）和定量逆转录聚合酶链反应，确定一组人类乳腺癌患者中转谷氨酰胺酶的表达水平及其可能的临床意义。正常乳腺组织通常低水平表达TGase-1、2、3和7，而高水平表达TGase-4、5和血浆TGase（因子ⅩⅢ）。与正常乳腺组织（n = 27）相比，肿瘤组织（n = 110）中TGase-4和7的转录水平显著升高，而因子ⅩⅢ的水平显著降低，p值分别为0.05、0.04和0.05。有淋巴结转移的肿瘤显示TGase-2水平显著升高，TGase-3水平降低（p值分别为0.05和0.046）。与无疾病复发的患者相比（中位随访72个月），转移性疾病患者中TGase-3和7的水平最低，死于乳腺癌的患者中TGase-3水平最低。局部复发患者中TGase-4和5的水平较高。乳腺癌表现出转谷氨酰胺酶的异常表达，其中TGase-2、3和7的水平与淋巴结受累情况及患者预后有关。
