Martin Tracey A, Gomez Kelvin, Watkins Gareth, Douglas-Jones Anthony, Mansel Robert E, Jiang Wen G
Metastasis and Angiogenesis Research Group, Wales College of Medicine, Cardiff University, Heath Park, Cardiff, CF4 4XN, UK.
Oncol Rep. 2006 Jul;16(1):207-12.
This study examined the expression and distribution of BCSG-1 in human breast cancer tissue. IHC revealed that BCSG-1 was primarily seen as a cytosolic protein, weakly staining normal mammary epithelial cells but increased in breast tumour cells. Q-PCR revealed that node negative and positive tumours had similar levels of BCSG-1 transcript and BCSG-1/CK19 ratio. There were significantly higher levels in grade 2 and grade 3 tumours compared to grade 1. Patients with NPI (Nottingham prognostic indicator) < 3.4, had a predicted 80% 15-year survival. After a 10-year follow-up, no significant difference was seen between tumours from patients remaining disease-free and those who died of breast cancer. The levels of BCSG-1 significantly correlated with an associated molecule, transglutaminase-3 (r = 0.307, P < 0.05), and weakly with transglutaminase-7 (r = 0.183). BCSG-1 is increased in breast tumour cells, is negatively associated with tumour grade and significantly correlates with levels of transglutaminase-3.
本研究检测了BCSG-1在人乳腺癌组织中的表达及分布情况。免疫组化显示，BCSG-1主要表现为一种胞质蛋白，在正常乳腺上皮细胞中染色较弱，但在乳腺肿瘤细胞中表达增加。定量聚合酶链反应显示，腋窝淋巴结阴性和阳性的肿瘤中BCSG-1转录本水平及BCSG-1/细胞角蛋白19比例相似。与I级肿瘤相比，II级和III级肿瘤中的水平显著更高。诺丁汉预后指数（NPI）<3.4的患者预计15年生存率为80%。经过10年随访，无病生存患者的肿瘤与死于乳腺癌患者的肿瘤之间未观察到显著差异。BCSG-1水平与相关分子转谷氨酰胺酶-3显著相关（r=0.307，P<0.05），与转谷氨酰胺酶-7弱相关（r=0.183）。BCSG-1在乳腺肿瘤细胞中表达增加，与肿瘤分级呈负相关，并与转谷氨酰胺酶-3水平显著相关。
