β-淀粉样前体蛋白抗体可识别阿尔茨海默病患者及对照成纤维细胞中的一种中间丝相关蛋白。

Antibody to beta-amyloid precursor protein recognizes an intermediate filament-associated protein in Alzheimer's and control fibroblasts.

作者信息

Dooley N P, Gauthier S, Durham H D

机构信息

Department of Neurology and Neurosurgery, McGill University, Montreal, Canada.

出版信息

J Neurosci Res. 1992 Sep;33(1):60-7. doi: 10.1002/jnr.490330108.

DOI:10.1002/jnr.490330108

PMID:1453484

Abstract

The expression of beta-amyloid precursor protein (BAPP) and its mRNAs was studied in fibroblasts obtained from patients afflicted with Alzheimer's disease (AD) and age-matched controls. Using reverse transcriptase-polymerase chain reaction (RT-PCR), transcripts corresponding to 770, 751, 714, and 695 amino acids were detected in both AD and control fibroblasts. Antibody 22C11 against BAPP (Boehringer Mannheim) labeled an intracellular protein, specifically localized to the intermediate filament network. In addition to bands of the predicted molecular weights for BAPP (120-135 kDa), Western blotting revealed a 57 kDa band which was not evident in samples of human brain. As cytoskeletal elements are vital in maintaining cellular architecture and various cell interactions, localization of BAPP or a related molecule to the cytoskeleton suggests a possible structural role for this protein within the cell.

摘要

在从阿尔茨海默病（AD）患者和年龄匹配的对照者获取的成纤维细胞中，研究了β-淀粉样前体蛋白（BAPP）及其mRNA的表达。使用逆转录聚合酶链反应（RT-PCR），在AD和成纤维细胞对照中均检测到对应于770、751、714和695个氨基酸的转录本。针对BAPP的抗体22C11（宝灵曼公司）标记了一种细胞内蛋白，该蛋白特异性定位于中间丝网络。除了BAPP预测分子量（120 - 135 kDa）的条带外，蛋白质印迹法还显示出一条57 kDa的条带，该条带在人脑样本中不明显。由于细胞骨架成分对于维持细胞结构和各种细胞相互作用至关重要，BAPP或相关分子定位于细胞骨架表明该蛋白在细胞内可能具有结构作用。