Uéda K, Cole G, Sundsmo M, Katzman R, Saitoh T
Department of Neurosciences, School of Medicine, University of California, San Diego, La Jolla 92093.
Ann Neurol. 1989 Mar;25(3):246-51. doi: 10.1002/ana.410250307.
The adhesiveness of fibroblasts derived from Alzheimer's disease (AD) patients to a plastic substratum was assessed as the proportion of cells attached to a plastic dish bottom after a 30-minute incubation in culture medium of cells dissociated in ethylenediaminetetraacetic acid and was compared with the adhesiveness of normal fibroblasts from non-AD controls. It was found that the normal fibroblasts adhered better to plastic than did AD cells. This reduced adhesiveness was observed in fibroblasts derived from both sporadic and familial AD patients. Because of the possible involvement of amyloid beta-protein precursor (ABPP) in the process of cellular adhesion, the amount of ABPP messenger RNA was measured in these fibroblasts and was found to be decreased in the familial AD fibroblasts, although not in cells from sporadic AD patients. Furthermore, there were fewer molecules detected by an anti-ABPP antibody in conditioned media from familial AD fibroblasts as compared with media from control fibroblasts. Therefore, although it is possible that the reduced adhesiveness of familial AD fibroblasts may result from genetic deficits in ABPP gene expression, the reduced adhesiveness of sporadic AD fibroblasts most likely results from certain deficits in molecules other than the ABPP.
将来自阿尔茨海默病（AD）患者的成纤维细胞与塑料基质的粘附性评估为在乙二胺四乙酸中解离的细胞在培养基中孵育30分钟后附着于塑料培养皿底部的细胞比例，并与来自非AD对照的正常成纤维细胞的粘附性进行比较。发现正常成纤维细胞比AD细胞更好地粘附于塑料。在散发性和家族性AD患者来源的成纤维细胞中均观察到这种粘附性降低。由于淀粉样β蛋白前体（ABPP）可能参与细胞粘附过程，因此在这些成纤维细胞中测量了ABPP信使RNA的量，发现家族性AD成纤维细胞中的ABPP信使RNA量减少，尽管散发性AD患者的细胞中没有减少。此外，与对照成纤维细胞的培养基相比，家族性AD成纤维细胞的条件培养基中用抗ABPP抗体检测到的分子更少。因此，虽然家族性AD成纤维细胞粘附性降低可能是由于ABPP基因表达的遗传缺陷所致，但散发性AD成纤维细胞粘附性降低最可能是由于ABPP以外的某些分子缺陷所致。
