Xu Tiefeng, Tang Kexuan, Zhang Hanming, Guo Meili, Zhang Lei, Li Fupeng
Pharmacy College, Second Military Medical University, Shanghai 200433.
Zhong Yao Cai. 2003 May;26(5):313-5.
The transgenic tetraploid of Isatis indigotica mediated by Agrobacterium tumefaciens was obtained. To transfer the plant binary expression vector pCAMBIA 3300 carrying bar gene, the Agrobacterium tumefaciens strain EHA 105 was used as engineering bacterium. The results of PCR indicated that the bar gene had been transferred into and merged with the genome of Isatis indigotica. This study will make foundation for improvement of other characters of this species with genetic engineering.
获得了根癌农杆菌介导的菘蓝转基因四倍体。为了转入携带bar基因的植物双元表达载体pCAMBIA 3300，使用根癌农杆菌菌株EHA 105作为工程菌。PCR结果表明bar基因已转入菘蓝基因组并与其融合。本研究将为利用基因工程改良该物种的其他性状奠定基础。
