Shi Qun, Tan Xiaodong, Mao Zongfu, Tao Ning, Yi Yourong
School of Public Health, Wuhan University, Wuhan 430071, China.
Wei Sheng Yan Jiu. 2003 Jul;32(4):381-3.
The extract solution from Sweet Potato was washed with 5% Na2CO3 and followed with distilled water and dried with dehydrated sodium sulfate (Na2SO4). The ether extract was evaporated and the residue (crude sample) was dissolved in a small volume of chloroform-soluble solution and was spotted on a silica gel G thin-layer plate developed with different solvent systems. Pure ipomeamarone and ipomeamaronol were used as standards. The resolution was the best in the solvent system of petroleum ether-ethylacetate at 20 degrees C. The separated spots were scanned by CS-930 TLC scanner at 520 nm and quantitative determination by external standard calibration. Ipomeamarone was detected by high performance thin-layer chromatography and the limits of detection were 0.002 microgram for pure and 0.01 microgram for crude sample.
红薯提取液先用5%碳酸钠洗涤，再用蒸馏水洗涤，然后用无水硫酸钠干燥。将乙醚提取物蒸发，残余物（粗样品）溶于少量氯仿可溶溶液中，并点样于用不同溶剂系统展开的硅胶G薄层板上。使用纯的甘薯黑疤霉酮和甘薯黑疤霉醇作为标准品。在20℃下，石油醚 - 乙酸乙酯溶剂系统中的分离效果最佳。分离出的斑点用CS - 930薄层扫描仪在520nm处扫描，并采用外标法进行定量测定。用高效薄层色谱法检测甘薯黑疤霉酮，纯品的检测限为0.002微克，粗样品的检测限为0.01微克。
