Hu M, Xiao P G
Yao Xue Xue Bao. 1989;24(12):923-31.
This paper reports a quantitative determination in the contents of flavonoids in Rhododendron leaves by HPTLC scanning method. The methanol extract of each sample was spotted on a HPTLC silica gel plate (E. Merck F254) alongside with standard substances. Using the upper layer of petroleum ether (60-90 degrees C)-ether-formic acid (62:31:7) as developing solvent I, farrerol, kaempferol and quercetin were well separated. The spots were determined by a single wavelength (366 nm) TLC-scanner, Camag model 76510. The same plate was then further developed by developing solvent II:7 ml of the lower layer of chloroform-methanol-water (7:3:1) plus 0.5 ml of formic acid to separate polystachoside, quercitrin and hyperoside. The densitometric determination of each spot was also carried out with the same scanner. The contents of 6 flavonoids in the leaves of 166 Rhododendron species were thus determined.
本文报道了采用高效薄层层析扫描法对杜鹃叶片中黄酮类化合物含量进行的定量测定。将每个样品的甲醇提取物与标准物质一起点样于高效薄层层析硅胶板（默克F254）上。以石油醚（60 - 90℃）-乙醚-甲酸（62:31:7）的上层作为展开剂I，法尔诺醇、山柰酚和槲皮素得到了良好分离。斑点用Camag 76510型单波长（366nm）薄层层析扫描仪进行测定。然后用展开剂II对同一薄板进一步展开：7ml氯仿-甲醇-水（7:3:1）的下层加0.5ml甲酸，以分离多穗花苷、槲皮苷和金丝桃苷。每个斑点的光密度测定也用同一台扫描仪进行。由此测定了166种杜鹃叶片中6种黄酮类化合物的含量。
