Parker Sarah, Campbell John, Gajadhar Alvin
Centre for Animal Parasitology, Saskatoon Laboratory, Canadian Food Inspection Agency, Saskatoon, Saskatchewan S7N 2R3, Canada.
J Vet Diagn Invest. 2003 Sep;15(5):460-5. doi: 10.1177/104063870301500510.
A number of different culture media have been described for use in the diagnosis of Tritrichomonas foetus infection in bulls, and recently, a commercial culture kit has become available. The objective of this study was to compare the sensitivity of 2 culture-based diagnostic tests for T. foetus in bulls. One test used a commercial kit for transport and culture of the samples. The other test used a thioglycollate transport medium (TFTM) for transport and a modified Diamond's medium (MDM) for culture of the samples. Twenty-one bulls infected with T. foetus were sampled repeatedly. On each sampling day, samples collected from the left and right sides of the bull were tested with one of the 2 diagnostic tests being compared. The effect of the type of diagnostic test on the outcome of the test was evaluated with a chi-square test for the calculated odds ratio. Because repeated tests from the same bull cannot be considered independent measures, unadjusted chi-square tests were adjusted for the effect of clustering by bull. Samples tested using the commercial kit were 6.95 times as likely to be positive as samples tested with a diagnostic test using MDM (P < 0.001).
已经描述了多种不同的培养基用于诊断公牛胎儿三毛滴虫感染，最近，一种商业培养试剂盒已经上市。本研究的目的是比较两种基于培养的公牛胎儿三毛滴虫诊断试验的敏感性。一种试验使用商业试剂盒进行样本的运输和培养。另一种试验使用硫乙醇酸盐运输培养基（TFTM）进行运输，并用改良的钻石培养基（MDM）进行样本培养。对21头感染胎儿三毛滴虫的公牛进行了多次采样。在每个采样日，从公牛左右两侧采集的样本用两种被比较的诊断试验之一进行检测。使用卡方检验计算比值比，评估诊断试验类型对试验结果的影响。由于来自同一头公牛的重复试验不能被视为独立测量，因此对未调整的卡方检验进行了调整，以考虑公牛聚类的影响。使用商业试剂盒检测的样本呈阳性的可能性是使用MDM诊断试验检测样本的6.95倍（P < 0.001）。
