Sydney School of Veterinary Science, Faculty of Science, University of Sydney,New South Wales, 2006,Australia.
Parasitology. 2019 Aug;146(9):1184-1187. doi: 10.1017/S0031182019000258. Epub 2019 Mar 12.
Bovine trichomoniasis is a notifiable, reproductive disease of cattle caused by the parasite Tritrichomonas foetus. Culturing with modified Diamond's medium (MDM) is required to increase the low number of organisms received from a preputial sample, but is limited in application to remote areas as it requires continuous cold chain storage. This study utilized lyophilization to sustain the viability of MDM during transport in lieu of a continuous cold chain. All lyophilized MDM was able to sustain T. foetus after storage for 42 days at 24 °C, and the results demonstrated that lyophilized MDM was equally as viable as refrigerated liquid MDM. Storage of lyophilized MDM at room temperature for 1 and 7 days did not impact T. foetus yield, both with and without exposure to light. A limitation of the lyophilized MDM was demonstrated with a significant decrease in T. foetus yield when the media was stored at 37 and 58 °C. The lyophilization of MDM provides a robust method of transporting and storing medium prior to reconstitution and inoculation, for use in T. foetus diagnosis and surveillance in remote areas.
牛滴虫病是一种由胎儿三毛滴虫引起的牛传染性生殖疾病。为了增加从包皮样本中获得的低数量的生物体，需要使用改良的戴蒙德培养基（MDM）进行培养，但由于其需要连续冷链储存，因此在应用上受到限制，只能在偏远地区使用。本研究利用冷冻干燥在运输过程中维持 MDM 的活力，以代替连续的冷链。所有冷冻干燥的 MDM 在 24°C 下储存 42 天后仍能维持胎儿三毛滴虫的存活，结果表明冷冻干燥的 MDM 与冷藏的液体 MDM 一样具有活力。冷冻干燥的 MDM 在室温下储存 1 天和 7 天，无论是在有光还是无光的情况下，都不会影响胎儿三毛滴虫的产量。冷冻干燥的 MDM 存在一个局限性，当培养基在 37 和 58°C 下储存时，胎儿三毛滴虫的产量会显著下降。MDM 的冷冻干燥为在重新配制和接种之前运输和储存培养基提供了一种可靠的方法，可用于在偏远地区进行胎儿三毛滴虫的诊断和监测。
