Roy Reena
St. Louis County Police Crime Laboratory, 111 South Meramec, Clayton, MO 63105, USA.
J Forensic Sci. 2003 Sep;48(5):1035-40.
Human stool samples from eight volunteers were stored under various conditions and extracted by three different procedures. Fecal material and tissue paper soiled with fecal material obtained from a crime scene were also extracted. Extracted DNA was amplified using the AmpFlSTR Profiler Plus, AmpFlSTR COfiler, and the AmpFlSTR Identifiler PCR amplification kits for the detection of the autosomal STR allelic patterns. DNA extracted from the male volunteers and from the soiled tissue paper evidence sample was also amplified using the Y-PLEX 6 and Y-PLEX 5 amplification kits. Analysis of the amplified products was carried out by capillary electrophoresis on the ABI PRISM 310 Genetic Analyzer. Autosomal and Y-STR profiles obtained from the fecal material were concordant with the results from the donors' buccal swabs.
来自八名志愿者的人类粪便样本在不同条件下储存，并通过三种不同程序进行提取。还提取了从犯罪现场获得的粪便物质以及被粪便物质弄脏的纸巾。使用AmpFlSTR Profiler Plus、AmpFlSTR COfiler和AmpFlSTR Identifiler PCR扩增试剂盒对提取的DNA进行扩增，以检测常染色体STR等位基因模式。从男性志愿者和弄脏的纸巾证据样本中提取的DNA也使用Y-PLEX 6和Y-PLEX 5扩增试剂盒进行扩增。扩增产物通过在ABI PRISM 310遗传分析仪上进行毛细管电泳进行分析。从粪便物质获得的常染色体和Y-STR图谱与供体口腔拭子的结果一致。
