Frégeau Chantal J, Bowen Kathy L, Leclair Benoît, Trudel Isabelle, Bishop Lucy, Fourney Ron M
Royal Canadian Mounted Police, Forensic Laboratory Services, National DNA Data Bank of Canada, 1200 Vanier Parkway, Ottawa, Ontario.
J Forensic Sci. 2003 Sep;48(5):1014-34.
As part of the validation of the AmpFlSTR Profiler Plus short tandem repeat (STR) system, under reduced polymerase chain reaction (PCR) volume conditions (i.e., 25 microL), a total of 275 casework samples were processed. Examples of profiles are presented along with amplification conditions to improve the odds of obtaining balanced and complete profiles for samples showing partial results or profiles with a descending slope. Data collected and used to develop our interpretation guidelines are included. From the mixture studies, full profiles were obtained for minor contributors, using 2 ng of DNA, with ratios of 10:1 or 1:20 and using 1 ng of DNA, with ratios of 10:1 and 1:8. The specificity of the Profiler Plus amplification reaction performed in 25 microL was examined and confirmed using a large spectrum of nonhuman DNAs. This report supports the use of the AmpFlSTR Profiler Plus STR system for casework DNA typing under reduced PCR volume conditions.
作为AmpFlSTR Profiler Plus短串联重复序列（STR）系统验证工作的一部分，在聚合酶链反应（PCR）体积减小的条件下（即25微升），共处理了275份实际案件样本。文中展示了图谱示例以及扩增条件，以提高对于呈现部分结果或图谱斜率下降的样本获得平衡且完整图谱的几率。还纳入了为制定我们的解释指南而收集和使用的数据。在混合样本研究中，对于次要贡献者，使用2纳克DNA、比例为10:1或1:20以及使用1纳克DNA、比例为10:1和1:8时，均获得了完整图谱。使用多种非人类DNA对在25微升体系中进行的Profiler Plus扩增反应的特异性进行了检测和确认。本报告支持在PCR体积减小的条件下，将AmpFlSTR Profiler Plus STR系统用于实际案件的DNA分型。
