一种由Y染色体短串联重复序列DYS441、DYS442、DYS443、DYS444和DYS445组成的新型多重聚合酶链反应系统。

A novel multiplex PCR system consisting of Y-STRs DYS441, DYS442, DYS443, DYS444, and DYS445.

作者信息

Iida Reiko, Sawazaki Kazumi, Ikeda Hitoe, Miyamoto Taito, Tsubota Etsuko, Takatsuka Hisakazu, Masuyama Mika, Matsuki Takasumi, Yasuda Toshihiro, Kishi Koichiro

机构信息

Department of Forensic Medicine, Fukui Medical University, Fukui 910-1193, Japan.

出版信息

J Forensic Sci. 2003 Sep;48(5):1088-90.

PMID:14535672

Abstract

We have developed a new sensitive multiplex PCR system consisting of five male-specific and polymorphic tetranucleotide STRs--DYS441 (GDB: 10013873), DYS442 (GDB: 10030304), DYS443 (GDB: 10807127), DYS444 (GDB: 10807128), and DYS445 (GDB: 10807129) on the Y chromosome. Fifty pg DNA per 10 microL reaction volume was required for the correct typing of five STRs. Using this system, the five Y-STRs were correctly typed from blood and semen stains that had been stored for several years at room temperature.

摘要

我们开发了一种新的灵敏多重PCR系统，该系统由Y染色体上五个男性特异性多态性四核苷酸STR组成，分别是DYS441（GDB：10013873）、DYS442（GDB：10030304）、DYS443（GDB：10807127）、DYS444（GDB：10807128）和DYS445（GDB：10807129）。每10微升反应体积需要50皮克DNA才能正确分型这五个STR。使用该系统，能从在室温下储存数年的血液和精液污渍中正确分型这五个Y-STR。