Aukema H M, Ogborn M R, Tomobe K, Takahashi H, Hibino T, Holub B J
Department of Nutritional Sciences, University of Guelph, Ontario, Canada.
Kidney Int. 1992 Oct;42(4):837-42. doi: 10.1038/ki.1992.358.
A paucity of research data exists on the potential for early dietary modification to directly retard cystic growth and proliferation in polycystic kidney disease (PKD). We have therefore examined the relative effects of dietary protein levels and oil type on the progression of disease in a murine model of PKD. In the first study, weanling DBA/2FG-pcy (pcy) mice were fed either a normal (NP), 25%, or low (LP), 6%, casein diet with 10% of either sunflower seed oil (SO) (containing n-6 fatty acids), or fish oil (FO) (containing n-3 fatty acids), in a 2 x 2 design. At the end of the dietary treatment, kidney weight relative to body weight was higher in mice on the NP diets. In addition, kidney phospholipid to kidney weight (mumol/g) was lower in pcy mice on NP diets, indicating that the increased kidney size was largely due to increased cyst development. Replacement of dietary SO with FO resulted in alterations in renal phospholipid fatty acid compositions: 18:2 n-6, 20:4 n-6, and 22:5 n-6 were lower, and 20:5 n-3, 22:5 n-3, and 22:6 n-3 were higher in FO-fed animals. No effect of dietary lipid type on disease progression was noted, however. In a second study, morphometric analysis revealed an 11% lower percentage cyst area and a 46% lower total cyst area (mm2) in kidney sections derived from mice on LP diets compared to NP diets. These results indicate that early dietary protein restriction in PKD prior to clinical manifestation of symptoms of the disease may have a significant impact on the pathogenesis of PKD.
关于早期饮食调整对多囊肾病（PKD）中囊肿生长和增殖的直接抑制作用，现有的研究数据很少。因此，我们研究了饮食蛋白质水平和油的类型对PKD小鼠模型中疾病进展的相对影响。在第一项研究中，将断奶的DBA/2FG-pcy（pcy）小鼠按照2×2设计，分别喂食正常（NP）、25%或低蛋白（LP）、6%的酪蛋白饮食，并添加10%的葵花籽油（SO，含n-6脂肪酸）或鱼油（FO，含n-3脂肪酸）。在饮食处理结束时，NP饮食组小鼠的肾脏重量相对于体重更高。此外，NP饮食组的pcy小鼠肾脏磷脂与肾脏重量的比值（μmol/g）更低，这表明肾脏体积增加主要是由于囊肿发育增加所致。用FO替代饮食中的SO导致肾脏磷脂脂肪酸组成发生变化：喂食FO的动物体内18:2 n-6、20:4 n-6和22:5 n-6含量降低，而20:5 n-3、22:5 n-3和22:6 n-3含量升高。然而，未观察到饮食脂质类型对疾病进展有影响。在第二项研究中，形态计量分析显示，与NP饮食组相比，LP饮食组小鼠肾脏切片中的囊肿面积百分比降低了11%，总囊肿面积（mm2）降低了46%。这些结果表明，在PKD症状临床表现出现之前，早期限制饮食蛋白质可能对PKD的发病机制有显著影响。
