Saffery Richard, Sumer Huseyin, Hassan Sara, Wong Lee H, Craig Jeffrey M, Todokoro Kazuo, Anderson Melissa, Stafford Angela, Choo K H Andy
The Murdoch Childrens Research Institute, Royal Children's Hospital, Flemington Road, Melbourne, 3052, Australia.
Mol Cell. 2003 Aug;12(2):509-16. doi: 10.1016/s1097-2765(03)00279-x.
Recent data in yeast and Drosophila suggest a domain-like centromere structure with a modified chromatin core and flanking regions of heterochromatin. We have analyzed a functional human centromere and defined a region of increased chromosome scaffold/matrix attachment that overlaps three other distinct and nonoverlapping domains for constitutive centromere proteins CENP-A and CENP-H, and heterochromatin protein HP1. Transcriptional competency is intact throughout the S/MAR-enriched region and within the CENP-A- and CENP-H-associated chromatin. These results provide insights into the relationship between centromeric chromatin and transcriptional competency in vivo, highlighting the permissibility of transcription within the constitutively modified, nonheterochromatic chromatin of a functional eukaryotic centromere.
酵母和果蝇的最新数据表明，着丝粒结构类似结构域，具有经过修饰的染色质核心和异染色质侧翼区域。我们分析了一个功能性人类着丝粒，并确定了一个染色体支架/基质附着增加的区域，该区域与组成型着丝粒蛋白CENP-A和CENP-H以及异染色质蛋白HP1的其他三个不同且不重叠的结构域重叠。在整个富含S/MAR的区域以及与CENP-A和CENP-H相关的染色质中，转录能力保持完整。这些结果为体内着丝粒染色质与转录能力之间的关系提供了见解，突出了在功能性真核生物着丝粒的组成型修饰、非异染色质染色质中转录的可能性。
