Glick B R, Bashan Y
Department of Biology, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1.
Biotechnol Adv. 1997;15(2):353-78. doi: 10.1016/s0734-9750(97)00004-9.
Plant growth-promoting bacteria (PGPB) control the damage to plants from phytopathogens by a number of different mechanisms including: outcompeting the phytopathogen, physical displacement of the phytopathogen, secretion of siderophores to prevent pathogens in the immediate vicinity from proliferating, synthesis of antibiotics, synthesis of a variety of small molecules that can inhibit phytopathogen growth, production of enzymes that inhibit the phytopathogen and stimulation of the systemic resistance of the plant. Biocontrol PGPB may be improved by genetically engineering them to overexpress one or more of these traits so that strains with several different anti-phytopathogen traits which can act synergistically are created. In engineering these strains it is essential to ensure that the normal functioning of the bacterium is not impaired, i.e., that there is no problem with metabolic load.
植物促生细菌（PGPB）通过多种不同机制控制植物病原体对植物的损害，这些机制包括：与植物病原体竞争、物理性取代植物病原体、分泌铁载体以防止附近病原体增殖、合成抗生素、合成多种可抑制植物病原体生长的小分子、产生抑制植物病原体的酶以及刺激植物的系统抗性。通过基因工程使生物防治PGPB过表达一种或多种这些特性，从而创造出具有几种可协同作用的不同抗植物病原体特性的菌株，可改进生物防治PGPB。在改造这些菌株时，必须确保细菌的正常功能不受损害，即代谢负荷没有问题。
