Instituto de Investigaciones Químico-Biológicas de la Universidad Michoacana de San Nicolás de Hidalgo, Morelia, Michoacán, Mexico; Department of Biology, University of Waterloo, Waterloo, Ontario, Canada.
Facultad de Agrobiología "Presidente Juárez", Universidad Michoacana de San Nicolás de Hidalgo, Uruapan, Michoacán, Mexico.
Microbiol Res. 2018 Mar;208:25-31. doi: 10.1016/j.micres.2018.01.005. Epub 2018 Jan 31.
A plant microbiome includes a microbial community that typically interacts extensively with a plant. The plant microbiome can survive either inside or outside of plant tissues, performing various plant beneficial activities including biocontrol of potential phytopathogens and promotion of plant growth. An important part of the plant microbiome includes plant growth-promoting bacteria (PGPB) that commonly reside in the rhizosphere and phyllosphere, and as endophytic bacteria (inside of plant tissues). As new plant microbiome-manipulating strategies have emerged in recent years, we have critically reviewed relevant literature, chiefly from the last decade. We have analysed and compared the rhizosphere, phyllosphere and endosphere as potential ecosystems for manipulation, in order to improve positive interactions with the plant. In addition, many studies on the bioengineering of the endophyte microbiome and its potential impact on the core microbiome were analysed with respect to five different strategies, including host mediated and multi-generation microbiome selection, inoculation into soil and rhizosphere, inoculations into seeds or seedlings, tissue atomisation and direct injection into tissues or wounds. Finally, microbiome engineering presents a feasible strategy to solve multiple agriculture-associated problems in an eco-friendly way.
植物微生物组包括一个微生物群落，通常与植物广泛相互作用。植物微生物组可以在植物组织内外生存，进行各种有益于植物的活动，包括对潜在植物病原体的生物防治和促进植物生长。植物微生物组的一个重要组成部分包括生长促进细菌（PGPB），它们通常存在于根际和叶际，以及作为内生细菌（在植物组织内部）。近年来，随着新的植物微生物组操纵策略的出现，我们对相关文献进行了批判性审查，主要来自过去十年。我们分析和比较了根际、叶际和内共生作为潜在的操纵生态系统，以改善与植物的积极相互作用。此外，还分析了内生微生物组的生物工程及其对核心微生物组的潜在影响的许多研究，涉及五个不同的策略，包括宿主介导和多代微生物组选择、接种到土壤和根际、接种到种子或幼苗、组织雾化和直接注射到组织或伤口。最后，微生物组工程提供了一种可行的策略，以生态友好的方式解决与农业相关的多个问题。
