梅毒诊断：通过酶免疫测定法进行筛查以及荧光密螺旋体抗体吸收试验（FTA - ABS）确证试验性能的差异

Syphilis diagnosis: screening by enzyme immunoassay and variation in fluorescent treponemal antibody absorbed (FTA-ABS) confirmatory test performance.

作者信息

Chronas G, Moyes A, Young H

机构信息

STD Diagnostic Laboratory, University of Edinburgh Medical School, Scotland, UK.

出版信息

Med Lab Sci. 1992 Mar;49(1):50-5.

PMID:1453909

Abstract

Four fluorescent treponemal antibody absorbed (FTA-ABS) test kits were evaluated with 86 treponemal and 84 non-treponemal sera selected with enzyme immunoassay (EIA). Agreement between all four kits was 63% for treponemal, and 50% for non-treponemal, sera. Discrepancies with treponemal sera were associated with low levels of antibody characterised by T. pallidum haemagglutination assay (TPHA) titres < or = 160 and a negative Venereal Diseases Research Laboratory (VDRL) test. Discrepancies with non-treponemal sera were significantly associated with false reactivity on screening with EIA. Possible reasons for the differences obtained between kits, the importance of screening policies for pre-selecting sera, and the significance of equivocal (borderline) reactions on positive and negative predictive values are discussed.

摘要

使用酶免疫测定法（EIA）从86份梅毒螺旋体血清和84份非梅毒螺旋体血清中选取样本，对四种荧光梅毒螺旋体抗体吸收试验（FTA-ABS）试剂盒进行了评估。对于梅毒螺旋体血清，四种试剂盒之间的一致性为63%；对于非梅毒螺旋体血清，一致性为50%。梅毒螺旋体血清的差异与梅毒螺旋体血凝试验（TPHA）滴度≤160且性病研究实验室（VDRL）试验为阴性所表征的低水平抗体有关。非梅毒螺旋体血清的差异与EIA筛查中的假反应性显著相关。讨论了各试剂盒之间出现差异的可能原因、血清预筛选策略的重要性以及阳性和阴性预测值中模棱两可（临界）反应的意义。