Squiers E C, Kelley S E, West J C
Department of Surgery, Geisinger Medical Center, Danville, PA 17822-0114.
Microsurgery. 1992;13(6):345-7. doi: 10.1002/micr.1920130612.
The rat has been used as a model to study the significance of graft and host interactions in small bowel transplantation (SBTX). A mouse model of SBTX would allow investigators to apply the knowledge of the well-defined genetics in the mouse to this field of study. Therefore, we have developed a mouse model of heterotopic SBTX using syngeneic C57BL6/J mice. Animals were anesthetized with a combination of ketamine and xylazine. Donor animals underwent midline laparotomy, with isolation of a segment of bowel as an isograft for transplantation to a recipient animal. The bowel was flushed in situ prior to removal of the graft with a Carrel patch of aorta and portal vein. The recipient animal underwent midline laparotomy and preparation of its infrarenal aorta and inferior vena cava for end-to-side anastomosis of the graft with 10-0 nylon. After vascular reperfusion of the graft the ends of the isografted bowel were brought out as stomata. Successful grafts were later assessed for viability by laparotomy or histological examination at the time of sacrifice. Areas of technical difficulty in this model and issues that might improve the experimental results are discussed. This model should allow investigators to apply the well-defined genetics of the mouse to probe the challenging field of intestinal transplantation.
大鼠已被用作研究小肠移植（SBTX）中移植物与宿主相互作用重要性的模型。SBTX的小鼠模型将使研究人员能够将小鼠中已明确的遗传学知识应用于该研究领域。因此，我们使用同基因C57BL6/J小鼠建立了异位SBTX小鼠模型。动物用氯胺酮和赛拉嗪联合麻醉。供体动物行中线剖腹术，分离一段肠作为同基因移植物移植到受体动物体内。在移除移植物之前，用带有主动脉和门静脉的卡雷尔补片对肠进行原位冲洗。受体动物行中线剖腹术，准备其肾下腹主动脉和下腔静脉，以便用10-0尼龙线将移植物进行端侧吻合。移植物血管再灌注后，将同基因移植肠的两端作为造口引出。成功的移植物随后在处死时通过剖腹术或组织学检查评估其活力。讨论了该模型中的技术难点以及可能改善实验结果的问题。该模型应使研究人员能够应用小鼠明确的遗传学来探索具有挑战性的肠道移植领域。
