大鼠肝移植的综合创新技术：手术指南。
Comprehensive and innovative techniques for liver transplantation in rats: a surgical guide.
机构信息
Department of Neuroscience, Mayo Clinic, Birdsall Research Building, Room 323, 4500 San Pablo Road, Jacksonville, Florida, FL 32224, United States.
出版信息
World J Gastroenterol. 2010 Jul 7;16(25):3120-32. doi: 10.3748/wjg.v16.i25.3120.
AIM
To investigate our learning curves of orthotopic liver transplantation (OLT) in rats and the most important factor for successful surgery.
METHODS
We describe the surgical procedures for our rat OLT model, and determined the operator learning curves. The various factors that contributed to successful surgery were determined. The most important surgical factors were evaluated between successful and unsuccessful surgeries.
RESULTS
Learning curve data indicated that 50 cases were required for operator training to start a study. Operative time, blood loss, warm ischemic time, anhepatic phase, unstable systemic hemodynamic state, and body temperature after surgery significantly affected surgery success by univariate analysis, while the anhepatic phase was the most critical factor for success by multivariate analysis.
CONCLUSION
OLT in rats is the only liver transplantation model that provides clinically relevant and reliable results. Shortened anhepatic phase is key to success in this model.
目的
探讨我们在大鼠原位肝移植（OLT）中的学习曲线和成功手术的最重要因素。
方法
我们描述了大鼠 OLT 模型的手术步骤，并确定了术者的学习曲线。确定了促成成功手术的各种因素。评估了成功手术和失败手术之间最重要的手术因素。
结果
学习曲线数据表明，需要进行 50 例手术训练才能开始研究。手术时间、失血量、热缺血时间、无肝期、全身血流动力学不稳定和术后体温通过单因素分析显著影响手术成功率，而无肝期是通过多因素分析确定的成功的最关键因素。
结论
大鼠 OLT 是唯一提供临床相关和可靠结果的肝移植模型。缩短无肝期是该模型成功的关键。
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