[作为载体pUC19一部分的链霉菌质粒pSG1912片段的克隆]
[The cloning of fragments of the streptomycete plasmid pSG1912 as a part of the vector pUC19].
作者信息
Zaverukha V B, Polevoda B V, Polishchuk L V, Matseliukh B P
出版信息
Mikrobiol Zh (1978). 1992 Sep-Oct;54(5):30-5.
PMID:1453989
Abstract
DNA fragments of plasmid pSG1912 isolated from Streptomyces globisporus 1912 have been cloned into vector pUC19 in the E. coli cells. Stability of inheritance of hybrid derivatives in enterobacteria has been studied. The clones where recombinant plasmids are stably inherited have been chosen. The detailed restriction maps of cloned DNA fragments of pSG1912 have been constructed.
摘要
从球孢链霉菌1912中分离出的质粒pSG1912的DNA片段已在大肠杆菌细胞中克隆到载体pUC19中。研究了杂种衍生物在肠道细菌中的遗传稳定性。选择了重组质粒能稳定遗传的克隆。构建了pSG1912克隆DNA片段的详细限制性图谱。
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