维氏硫杆菌隐蔽质粒pTAV-1 DNA在大肠杆菌中的克隆与表达。
Cloning and expression of Thiobacillus versutus cryptic plasmid pTAV-1 DNA in Escherichia coli.
作者信息
Jagusztyn-Krynicka E K, Bielecki J, Kauc B
机构信息
Institute of Microbiology, University of Warsaw, Poland.
出版信息
Microbios. 1990;61(246):49-61.
PMID:2179679
Abstract
pTAV-1 is an approximately 100 kb Thiobacillus versutus cryptic plasmid. pTAV-1 DNA was cloned in Escherichia coli. Nine recombinant plasmids containing pTAV-1 DNA inserted into the EcoRI restriction site of pACYC184 were constructed. The origin of DNA inserts was confirmed by Southern blot hybridization. The expression of mixotrophic T. versutus plasmid genes was demonstrated in E. coli.
摘要
pTAV - 1是一种约100 kb的维氏硫杆菌隐蔽质粒。pTAV - 1 DNA在大肠杆菌中进行了克隆。构建了九个重组质粒，这些质粒将pTAV - 1 DNA插入到pACYC184的EcoRI限制位点。通过Southern印迹杂交确认了DNA插入片段的来源。在大肠杆菌中证实了兼性营养型维氏硫杆菌质粒基因的表达。
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