用于节杆菌菌株中2,6 - 二甲基吡啶、2,4 - 二甲基吡啶和吡啶生物降解的质粒

[Plasmids for biodegradation of 2,6-dimethylpyridine, 2,4-dimethylpyridine, and pyridine in strains of Arthrobacter].

作者信息

Agapova S R, Andreeva A L, Starovoĭtov I I, Vorob'eva L I, Terent'ev P B

出版信息

Mol Gen Mikrobiol Virusol. 1992 May-Jun(5-6):10-3.

Abstract

Arthrobacter crysallopoietes strain KM-4 degrading 2,6-dimethylpyridine and strain KM-4a degrading both 2,6-dimethylpyridine and pyridine, Arthrobacter sp. KM-4b degrading 2,4-dimethylpyridine were isolated from soil. Arthrobacter crystallopoietes KM-4 and Arthrobacter sp. KM-4b contain 100 Md plasmids pBS320 and pBS323. Arthrobacter crystallopietes KM-4a harbours a 100 Md and 80 Md plasmids. Plasmid curing and conjugation transfer results confirm that these plasmids are involved in degradation of 2,6-dimethylpyridine, 2,4-dimethylpyridine and pyridine. A mutant with lost ability to degrade 2,6-dimethylpyridine was isolated during the growth of strain KM-4 rifR at 42 degrees C. Electrophoretic analysis of the plasmid from temperature sensitive mutant revealed the deletion the size of 26 Md from pBS320 plasmid.

摘要

从土壤中分离出了能降解2,6 - 二甲基吡啶的结晶节杆菌菌株KM - 4、能降解2,6 - 二甲基吡啶和吡啶的菌株KM - 4a以及能降解2,4 - 二甲基吡啶的节杆菌属菌株KM - 4b。结晶节杆菌KM - 4和节杆菌属菌株KM - 4b含有100 Md的质粒pBS320和pBS323。结晶节杆菌KM - 4a含有一个100 Md和一个80 Md的质粒。质粒消除和接合转移结果证实这些质粒参与了2,6 - 二甲基吡啶、2,4 - 二甲基吡啶和吡啶的降解。在菌株KM - 4 rifR于42℃生长期间，分离出了一株丧失降解2,6 - 二甲基吡啶能力的突变体。对来自温度敏感突变体的质粒进行电泳分析，结果显示pBS320质粒缺失了26 Md大小的片段。