Reikofski J, Tao B Y
Biochemical and Food Process Engineering, Department of Agricultural Engineering, Purdue University, West Lafayette, IN, USA.
Biotechnol Adv. 1992;10(4):535-47. doi: 10.1016/0734-9750(92)91451-j.
Polymerase chain reaction (PCR) technology has revolutionized the process of isolating and amplifying segments of DNA. One powerful application of PCR is its use in precise site-directed mutagenesis (SDM). SDM provides an elegant tool for scientists and engineers to explore biocatalytic mechanisms and processes to understand the structural-functional relationships of enzymes and other proteins. This article reviews techniques and methodology used in site-directed mutagenesis of genes by PCR.
聚合酶链反应（PCR）技术彻底改变了DNA片段的分离和扩增过程。PCR的一个强大应用是其在精确的定点诱变（SDM）中的应用。SDM为科学家和工程师提供了一个精妙的工具，用于探索生物催化机制和过程，以了解酶和其他蛋白质的结构-功能关系。本文综述了通过PCR进行基因定点诱变所使用的技术和方法。
