Weiss B, Sachs L
J Cell Physiol. 1977 Nov;93(2):183-8. doi: 10.1002/jcp.1040930203.
The hydrolysis of ATP and AMP by enzymes located on the external side of the plasma membrane (ecto-ATPase and ecto-AMPase) was studied in mouse myeloid leukemic cells, normal early myeloid cells, and normal mature granulocytes and macrophages. Nine clones of myeloid leukemic cells were used belonging to three groups that differ in their ability to be induced to differentiate by the differentiation-inducing protein MGI. These three groups consisted of MGI+D+ that can be induced to undergo complete differentiation, MGI+D- that can be induced to partially differentiate and MGI-D- with no induction of differentiation. The ecto-ATPase activity of normal early myeloid cells was similar to that of normal mature granulocytes and macrophages and higher than that of any of the leukemic cells. Among the leukemic cells, the MGI-D- cells had the highest level of ecto-ATPase activity. The behaviour of ecto-AMPase differed from that of ecto-ATPase. Some MGI-D- clones had a higher ecto-AMPase activity than normal cells and MGI+D- and MGI+D+ cells showed no detectable activity. Neither the ecto-ATP-ase nor ecto-AMPase activities changed after induction of differentiation in normal early myeloid or MGI+D+ leukemic cells. The results indicate that the myeloid leukemic cells had a decreased ability to hydrolyse external ATP, that there can be an independent regulation of ecto-ATPase and ecto-AMPase and that neither of these enzyme activities changed during differentiation.
在小鼠髓系白血病细胞、正常早期髓系细胞以及正常成熟粒细胞和巨噬细胞中，研究了位于质膜外侧的酶（胞外ATP酶和胞外AMP酶）对ATP和AMP的水解作用。使用了九个髓系白血病细胞克隆，它们分属于三组，这三组在被分化诱导蛋白MGI诱导分化的能力上有所不同。这三组包括可被诱导进行完全分化的MGI+D+组、可被诱导进行部分分化的MGI+D-组以及未被诱导分化的MGI-D-组。正常早期髓系细胞的胞外ATP酶活性与正常成熟粒细胞和巨噬细胞的相似，且高于任何白血病细胞。在白血病细胞中，MGI-D-细胞的胞外ATP酶活性水平最高。胞外AMP酶的表现与胞外ATP酶不同。一些MGI-D-克隆的胞外AMP酶活性高于正常细胞，而MGI+D-和MGI+D+细胞未显示出可检测到的活性。在正常早期髓系细胞或MGI+D+白血病细胞中诱导分化后，胞外ATP酶和胞外AMP酶活性均未改变。结果表明，髓系白血病细胞水解胞外ATP的能力下降，胞外ATP酶和胞外AMP酶可能存在独立调节，且在分化过程中这两种酶的活性均未改变。
