Tupper J T
J Cell Physiol. 1977 Nov;93(2):303-7. doi: 10.1002/jcp.1040930216.
Unindirectional potassium influx and the fraction of this influx sensitive to ouabain, an inhibitor of the (Na + K) activated ATPase, have been evaluated as a function of subcultivation of the 3T3 and SV40 transformed 3T3 cell. Total and ouabain-sensitive K influx change little over approximately 50 passages of the transformed 3T3 cell. In contrast, these components of K influx increase nearly 5-fold over a similar number of passages of the 3T3 cell. During early passages total and ouabain-sensitive K influx of the 3T3 cell are below that of the SV40 3T3 cell on a per cell volume basis. At later passages the magnitude of these components of K transport exceed those found in the SV40 3T3 cell. Previous studies have reported the ouabain-sensitive uptake of K and the levels of (Na + K) activated ATPase as being higher, lower or equivalent in the 3T3 versus transformed 3T3 cell. The present data suggest these differences may results from the degree to which the cells were passaged at the time of the experiments. Evaluation of previous studies substantiates this conclusion.
单向钾流入以及该流入中对哇巴因（一种（钠+钾）激活的ATP酶抑制剂）敏感的部分，已被评估为3T3细胞和SV40转化的3T3细胞传代培养的函数。在转化的3T3细胞大约50次传代过程中，总的和对哇巴因敏感的钾流入变化很小。相比之下，在3T3细胞类似次数的传代过程中，钾流入的这些成分增加了近5倍。在早期传代时，按每细胞体积计算，3T3细胞总的和对哇巴因敏感的钾流入低于SV40 3T3细胞。在后期传代时，钾转运的这些成分的幅度超过了SV40 3T3细胞中的幅度。先前的研究报告称，3T3细胞与转化的3T3细胞相比，对哇巴因敏感的钾摄取以及（钠+钾）激活的ATP酶水平更高、更低或相当。目前的数据表明，这些差异可能是由于实验时细胞传代的程度所致。对先前研究的评估证实了这一结论。
