血清通过增加钠离子内流来刺激静止成纤维细胞中的钠钾泵。
Serum stimulates the Na+,K+ pump in quiescent fibroblasts by increasing Na+ entry.
作者信息
Smith J B, Rozengurt E
出版信息
Proc Natl Acad Sci U S A. 1978 Nov;75(11):5560-4. doi: 10.1073/pnas.75.11.5560.
Abstract
Two ionophores (monensin and gramicidin) that carry Na+ into 3T3 cells markedly enhance the rate of 86Rb+ uptake. Ouabain prevents both ionophores from increasing 86Rb+ uptake, indicating that the ionophores activate the Na+,K+ pump. Measurements of 86Rb+ uptake and cell Na+ and K+ over a range of monensin concentrations show that the activity of the Na+,K+ pump in 3T3 cells is limited by the supply of internal Na+ and is extremely sensitive to small changes in internal Na+. Serum rapidly enhances the rate of 22Na+ uptake and net Na+ entry when Na+ exit is inhibited by ouabain. At 0.3 microgram/ml, monensin increases the rate of net Na+ entry and activates the Na+,K+ pump by the same degree as serum. The stimulation of 86Rb+ uptake by serum or the ionophores has an absolute requirement for external Na+. Thus, serum appears to stimulate the Na+,K+ pump in quiescent 3T3 cells by increasing its supply of Na+.
摘要
两种能将钠离子转运至3T3细胞内的离子载体（莫能菌素和短杆菌肽）显著提高了86铷离子的摄取速率。哇巴因可阻止这两种离子载体增加86铷离子的摄取，这表明离子载体激活了钠钾泵。在一系列莫能菌素浓度下对86铷离子摄取以及细胞内钠钾离子的测量结果显示，3T3细胞中钠钾泵的活性受细胞内钠离子供应的限制，并且对细胞内钠离子的微小变化极为敏感。当哇巴因抑制钠离子外流时，血清能迅速提高22钠离子的摄取速率和净钠离子内流。在0.3微克/毫升的浓度下，莫能菌素增加净钠离子内流的速率并激活钠钾泵，其程度与血清相同。血清或离子载体对86铷离子摄取的刺激绝对依赖于细胞外的钠离子。因此，血清似乎通过增加钠离子供应来刺激静止状态下3T3细胞中的钠钾泵。
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Proc Natl Acad Sci U S A. 1978 Nov;75(11):5560-4. doi: 10.1073/pnas.75.11.5560.
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本文引用的文献
1
Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines.对培养的小鼠胚胎细胞生长及其发育成既定细胞系的定量研究。
J Cell Biol. 1963 May;17(2):299-313. doi: 10.1083/jcb.17.2.299.
2
Sodium transport across the surface membrane of red blood cells in hereditary spherocytosis.遗传性球形红细胞增多症中钠在红细胞表面膜的转运
J Clin Invest. 1957 Jun;36(6 Part 1):816-24. doi: 10.1172/JCI103487.
3
Congenital hemolytic anemia with high sodium, low potassium red cells. I. Studies of membrane permeability.伴有高钠低钾红细胞的先天性溶血性贫血。I. 膜通透性研究。
N Engl J Med. 1968 Mar 14;278(11):573-81. doi: 10.1056/NEJM196803142781101.
4
The interaction of sodium and potassium with the sodium pump in red cells.钠和钾与红细胞中钠泵的相互作用。
J Physiol. 1973 Jun;231(2):297-325. doi: 10.1113/jphysiol.1973.sp010234.
5
Evidence for the genetic control of the sodium pump density in HeLa cells.HeLa细胞中钠泵密度遗传控制的证据。
J Physiol. 1974 Sep;241(3):771-94. doi: 10.1113/jphysiol.1974.sp010684.
6
Inheritance of an increased sodium pump in human red cells.人类红细胞中钠泵增加的遗传现象。
Nature. 1969 Mar 29;221(5187):1222-4. doi: 10.1038/2211222a0.
7
Interaction of two mechanisms regulating alkali cations in HeLa cells.HeLa细胞中两种调节碱金属阳离子机制的相互作用。
J Cell Physiol. 1975 Aug;86(1):59-70. doi: 10.1002/jcp.1040860108.
8
Control of growth of mammalian cells in cell culture.细胞培养中哺乳动物细胞生长的控制
Nature. 1975 Dec 11;258(5535):487-90. doi: 10.1038/258487a0.
9
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Proc Natl Acad Sci U S A. 1975 Nov;72(11):4492-5. doi: 10.1073/pnas.72.11.4492.
10
Control of protein synthesis in human fibroblasts by intracellular potassium.细胞内钾对人成纤维细胞蛋白质合成的调控
Exp Cell Res. 1977 Mar 15;105(2):223-36. doi: 10.1016/0014-4827(77)90120-3.
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