Buchman V L, Ninkina N N, Bogdanov Y D, Bortvin A L, Akopian H N, Kiselev S L, Anokhin K V, Georgiev G P
Engelhardt Institute of Molecular Biology, Moscow, Russia.
Nucleic Acids Res. 1992 Nov 11;20(21):5579-85. doi: 10.1093/nar/20.21.5579.
We have cloned a novel neurospecific gene, named neuro-d4, by differential screening a rat cerebral cortex cDNA library. Northern blot hybridization showed that neuro-d4 expression is restricted to neuronal tissues both in newborn and adult animals. The level of neuro-d4 mRNA in the rat central nervous system is high during the later stages of embryonic development and gradually decreases during the postnatal period. In situ hybridization suggests that the gene transcripts are localized in neuronal cell bodies. Nucleotide sequences of overlapped cDNA clones and all 12 exons in genomic clone were determined. The deduced protein has consensus sequences for a nuclear localization signal, a Krüppel-type zinc-finger and a new type of cysteine/histidine-rich motif resembling zinc-fingers. Several differential splicing variants were found, each of which influences the structure of the encoded protein.
我们通过差异筛选大鼠大脑皮质cDNA文库，克隆了一个名为neuro-d4的新型神经特异性基因。Northern印迹杂交显示，在新生和成年动物中，neuro-d4的表达均局限于神经组织。大鼠中枢神经系统中neuro-d4 mRNA的水平在胚胎发育后期较高，在出生后逐渐降低。原位杂交表明该基因转录本定位于神经元细胞体。测定了重叠cDNA克隆的核苷酸序列以及基因组克隆中所有12个外显子的序列。推导的蛋白质具有核定位信号、Krüppel型锌指和一种类似于锌指的新型富含半胱氨酸/组氨酸基序的共有序列。发现了几种可变剪接变体，每种变体都影响编码蛋白的结构。
